Composition of the ferredoxin-nitrite reductase from the cyanobacterium Phormidium laminosum.

Kidneys flush-stored with M C were compared with those in U W for 2 4 h and 48 h. At both 24 h and 48 h of storage, UW-stored organs produced si nificantly more glucose than those in MC. T h e uptake of "!I-PAH did not differ significantly between the two solutions. U W was subsequently modified, replacing raffinose with mannitol (solution A), lactobionate with citric acid (solution B), and sodium and potassium citrate (solution C). Hydroxyethyl starch, penicillin and dexamethasone were omitted from all three. Gluconeogenesis was significantly higher in the kidneys flushed in solution C (after incubation in Krebs-Ringer bicarbonate and Krebs-Ringer bicarbonate plus pyruvate) when compared with the other two solutions and the complete U W at both periods of storage (Fig. 1). IZSI-PAH uptake was also highest for solution C at 25 h and 48 h. These results showed that solution C was capable of retaining metabolic function in the kidneys better than the other solutions. These two metabolic function tests have been used to demonstrate persisting activity in flush-stored kidneys. Previous studies have validated these tests in terms of ultrastructural integrity, as seen by electron microscopy [8, 91 in the case of gluconeogenesis and other renal function tests in the case of 1251-PAH uptake [4, 51. This study has shown that simple metabolic function tests can be employed to determine activity in flush-stored kidneys and in addition can demonstrate differences in protection of certain metabolic pathways in stored renal tissue resulting from use of different flush solutions. Solution C warrants further investigation for renal preservation and may provide a cheaper alternative to other clinical flush solutions.